Project leader training at Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France// Radni posjet suradnoj instituciji na projektu

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Gabriela Vuletin Selak, Ph.D, from Institute for Adriatic Crops and Karst Reclamation, Split, has started her two months training at Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France, starting from 9 June to 11 August 2018. In collaboration with Petar Pujic, PhD, she will work on meeting two main objectives using the set of experiments proposed by the UKF project. The objectives are:

Objective 1. To detect the role of LTPs (lipid transfer proteins) in pollen tube growth and guidance. This objective will be met through the in vitro assay using the purified stigma protein to study the role of LTP in pollen tube growth and guidance.

Objective 2. To determine the effectiveness of the flower structures in directing the pollen tubes by locating the LTPs in the pistils (chemotropic effect) by immunohistochemically methods and specific antibodies for LTPs.

Two additional set of experiment will be performed to support the main objectives of the project: (i) The expression analysis of germinating pollen grains and pistils by transcriptomic analysis using RNAseq with the aim to identify specific expressed genes in pollen during germination and in pistil at anthesis and (ii) shotgun proteomics (LC/MS/MS analysis) in identifying proteins of pollen and pistil samples.

 

 

 

 

 

Dr. sc. Gabriela Vuletin Selak s Instituta za jadranske kulture i melioraciju krša u Splitu, započela je svoj dvomjesečni boravak u instituciji suvoditelja projekta na Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France u trajanju od 6. lipnja do 11. kolovoza 2018. U suradnji s dr. sc. Petrom Pujićem radit će na ostvarivanju glavnih ciljeva UKF projekta: (i) određivanju uloge LTP proteina (engl. lipid transfer proteins) u rastu i usmjeravanju peludnih mješinica (in vitro eksperimenti) te (ii) određivanju uloge pojedinih struktura u cvijetu u usmjeravanju peludnih mješinica lokalizacijom LTP proteina pri tom se koristeći imunohistokemijskim metodama i antitijelima za LTP proteine iz masline.