Gabriela Vuletin Selak, Ph.D. held a lecture entitled ʽNew approaches in understanding of the compatibility relationships and pollen–pistil interactions in olive (Olea europaea L.)ʼ at Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France as a visiting researcher of the team 1 Ecologie Microbienne. Lecture was held on 9 July 2018 in the Mendel Library of the University.

Dr. sc. Gabriela Vuletin Selak s Instituta za jadranske kulture je održala predavanje naslova ʽNovi pristupi u razumijevanju reproduktivne kompatibilnosti i interakcije pelud–tučak kod masline (Olea europaea L.)ʼ kao gostujući istraživač na instituciji Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon u Francuskoj. Predavanje je održano u biblioteci Sveučilišta u Lyonu 9. srpnja 2018.

Gabriela Vuletin Selak, Ph.D. held a seminar entitled ʽReproductive biology of olive: compatibility relationships between cultivars’ at Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France as a visiting researcher of the team Ecologie Microbienne. Seminar was held on 3 July 2018 in the Mendel Library of the University.

Dr. sc. Gabriela Vuletin Selak s Instituta za jadranske kulture je održala seminar naslova ʽReproduktivna biologija masline: odnosi kompatibilnosti među sortamaʼ kao gostujući istraživač na instituciji Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon u Francuskoj. Seminar je održan u biblioteci Sveučilišta u Lyonu 3. srpnja 2018.

Gabriela Vuletin Selak, Ph.D, from Institute for Adriatic Crops and Karst Reclamation, Split, has started her two months training at Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France, starting from 9 June to 11 August 2018. In collaboration with Petar Pujic, PhD, she will work on meeting two main objectives using the set of experiments proposed by the UKF project. The objectives are:

Objective 1. To detect the role of LTPs (lipid transfer proteins) in pollen tube growth and guidance. This objective will be met through the in vitro assay using the purified stigma protein to study the role of LTP in pollen tube growth and guidance.

Objective 2. To determine the effectiveness of the flower structures in directing the pollen tubes by locating the LTPs in the pistils (chemotropic effect) by immunohistochemically methods and specific antibodies for LTPs.

Two additional set of experiment will be performed to support the main objectives of the project: (i) The expression analysis of germinating pollen grains and pistils by transcriptomic analysis using RNAseq with the aim to identify specific expressed genes in pollen during germination and in pistil at anthesis and (ii) shotgun proteomics (LC/MS/MS analysis) in identifying proteins of pollen and pistil samples.

Dr. sc. Gabriela Vuletin Selak s Instituta za jadranske kulture i melioraciju krša u Splitu, započela je svoj dvomjesečni boravak u instituciji suvoditelja projekta na Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France u trajanju od 6. lipnja do 11. kolovoza 2018. U suradnji s dr. sc. Petrom Pujićem radit će na ostvarivanju glavnih ciljeva UKF projekta: (i) određivanju uloge LTP proteina (engl. lipid transfer proteins) u rastu i usmjeravanju peludnih mješinica (in vitro eksperimenti) te (ii) određivanju uloge pojedinih struktura u cvijetu u usmjeravanju peludnih mješinica lokalizacijom LTP proteina pri tom se koristeći imunohistokemijskim metodama i antitijelima za LTP proteine iz masline.

At the Institute for Adriatic Crops, during the visit of Dr. Petar Pujić, the UKF project co-leader and the respectable scientist from the Centre national de la recherche scientifique (UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Université Lyon 1, Lyon, France), the Croatian Radio Television (HRT), the national public service broadcaster of the Republic of Croatia reported about our UKF project at HRT 1 channel during Dobro jutro, Hrvatska (“Good morning, Croatia”), mosaic morning show.

Na institutu za jadranske kulture i melioraciju krša, tijekom boravka dr. Petra Pujića, suvoditelja UKF projekta i uglednog znanstvenika iz Francuskog nacionalnog centara za znanost, Laboratorija za mikrobnu ekologiju, Lyon, Francuska (UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Université Lyon 1, Lyon, France), Hrvatska radiotelevizija (HRT) je izvijestila o našem projektu, posjetu dr. Pujića, radionici i sastanku svih partnera na projektu tijekom popularne HRT-ove mozaične emisije Dobro jutro, Hrvatska u prvom programu HRT.

The workshop entitled “Research methodology in soil, microbial and plant systems” within the UKF project ʽNew approaches in understanding of the compatibility relationships and pollen–pistil interactions in olive (Olea europaea L.)ʼ was held on May 22 in the Institute for Adriatic Crops and Karst Reclamation. Co-workers at the project from all partners’ institutions participated in the workshop and the project meeting.

During the workshop the following lectures were presented to audience:

1. New research methodology in assessment of the compatibility relationships and pollen–pistil interactions (Gabriela Vuletin Selak, Institute for Adriatic Crops and Karst Reclamation, Croatia)
2. Efficient DNA extraction from olive (Olea europaea L.) embryos for parentage analysis (Alenka Baruca Arbeiter, University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies, Slovenia)
3. The intimate life of Actinorhizal plants and symbiotic Actinobacteria (Petar Pujic, Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France

The implementation of the first part of the project was discussed at the beginning of the project personnel meeting. Gabriela Vuletin Selak reported about the accomplished milestones and KPIs and certain financial questions.

Working plan for the second project period was discussed, where further work on leaf, flower, pollen and embryo samples was planned. Participation in scientific symposiums, publishing of scientific and professional publications, application to other sources of funds is also planned as well as scientific training of project leader and laboratory analyses of the samples collected at the second field experiment.

Radionica naziva „Znanstvena metodologija u sustavima tla, mikroorganizama i biljaka“ u  sklopu UKF projekta „Novi pristupi u razumijevanju reproduktivne kompatibilnosti i interakcije pelud–tučak kod masline (Olea europaea L.)“, održana je u Splitu, 22. svibnja 2018. u Institutu za jadranske kulture i melioraciju krša.

Tijekom radionice, sljedeća predavanja su predstavljena znanstvenoj publici:

1. Nova metodologija u izučavaju reproduktivne kompatibilnosti i interakcije pelud–tučak (Gabriela Vuletin Selak, Institut za jadranske kulture i melioraciju krša, Hrvatska)
2. Učinkovita izolacija DNA iz embrija masline (Olea europaea L.) u svrhu određivanja roditeljstva (Alenka Baruca Arbeiter, University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies, Slovenija)
3. Intimni život aktinoriznih biljaka i simbiotskih aktinobakterija (Petar Pujic, Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, Francuska)

Tijekom sastanka projektnog tima, raspravljena je provedba prvog dijela projekta. Gabriela Vuletin Selak je izvijestila o izvršenim miljokazima, ostvarenim ključnim pokazateljima provedbe projekta (KPI) i financijskim sredstvima za prvo razdoblje trajanja projekta.

Raspravljeno je o radnom planu za drugi dio projekta, gdje su planirane daljnje analize prikupljenog biljnog materijala. Iznesen je plan sudjelovanja na znanstvenim skupovima, publiciranja rezultata u znanstvenim i stručnim časopisima, prijavljivanja na druge izvore financiranja te odlazak voditelja projekta na znanstveno usavršavanje u instituciju suvoditelja projekta dr. Pujića u svrhu analiziranja uzoraka prikupljenih tijekom drugog eksperimenta u razdoblju cvjetanja.

Split, 22 May 2018. (9:00)

This is an announcement of the workshop “Research methodology in soil, microbial and plant systems” within the UKF project ʽNew approaches in understanding of the compatibility relationships and pollen–pistil interactions in olive (Olea europaea L.)ʼ

The workshop is intended for project personnel and general audience.

Programme

1. New research methodology in assessment of the compatibility relationships and pollen–pistil interactions

Gabriela Vuletin Selak, Institute for Adriatic Crops and Karst Reclamation, Croatia

2. Efficient DNA extraction from olive (Olea europaea L.) embryos for parentage analysis

Alenka Baruca Arbeiter, University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies, Slovenia

3. The intimate life of Actinorhizal plants and symbiotic Actinobacteria

Petar Pujic, Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, France

***

Split, 22. svibnja 2018., s početkom u 9:00 u Maloj vijećnici Instituta za jadranske kulture

U sklopu UKF projekta Novi pristupi u razumijevanju reproduktivne kompatibilnosti i interakcije pelud–tučak kod masline (Olea europaea L.), održat će se radionica naziva Znanstvena metodologija u sustavima tla, mikroorganizama i biljaka.

Radionica je namijenjena suradnicima s UKF projekta i široj znanstvenoj publici.

U sklopu radionice održat će se sljedeća predavanja:

1. Nova metodologija u izučavaju reproduktivne kompatibilnosti i interakcije pelud–tučak

Gabriela Vuletin Selak, Institut za jadranske kulture i melioraciju krša, Hrvatska

2. Učinkovita izolacija DNA iz embrija masline (Olea europaea L.) u svrhu određivanja roditeljstva

Alenka Baruca Arbeiter, University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies, Slovenija

3. Intimni život aktinoriznih biljaka i simbiotskih aktinobakterija

Petar Pujic, Université Lyon 1, UMR CNRS 5557 Ecologie Microbienne, UMR INRA 1418 – VetAgro Sup, Lyon, Francuska

Activities:

1. Assessment of the flowering periods
2. Collection of the samples of pistils for histology and immunohistochemistry analyses, and pollen for in vitro assay. Perfect flowers from ‘Oblica’ trees were gathered periodically at stages just before the anthesis, during the flowering period and at the end of this period. Pollen samples were collected at the full bloom.
3. The samples are stored in 4% Paraformaldehyde Solution in PBS; and another set at – 70 °C until further analysis in the laboratories of the University of Lyon and the University of Split.Aktivnosti:
   1. Praćenje razdoblje cvjetanja sorti
   2. Prikupljanje uzoraka cvjetova (tučkova) tijekom različitih razvojnih stadija
   3. Pohranjivanje uzoraka u 4% paraformaldehidu u PBS; te dijela uzoraka na – 70°C do predstojećih analiza u laboratorijima Sveučilišta u Lyonu i Sveučilišta u Splitu.

   

   

Based on the Agreement between two governments on scientific and technological collaboration, from 1997 to 2018, 16 sessions of the Joint Commission for Scientific and Technological Collaboration were held. At the sixteenth meeting of the Joint Commission, held on March 10, 2018 in Ljubljana, 52 new research projects have been approved for the period 2018 – 2019. The project entitled “Application of biotechnology tools for applied research in the Mediterranean agriculture: introduction of paternity tests in genotyping plants” and led by Professor Andreja Kutnar (University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies – Koper, Slovenia) and dr. Gabriela Vuletin Selak (Institute for Adriatic Crops and Karst Reclamation – Split, Croatia) was approved for financing. Professor Dunja Bandelj, dr. Alenka Baruca Arbeiter, dr. Slavko Perica and dr. Tatjana Klepo, members of the research group at the UKF project are participating in a new project too. In this new project olive and pomegranate are the species of our interest.

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Odobren novi bilateralni hrvatsko-slovenski znanstveno-istraživački projekt na kojem sudjeluju znanstvenici s UKF projekta

Na temelju Sporazuma dviju vlada o znanstvenoj i tehnološkoj suradnji, od 1997. do 2018. godine održano je 16 zasjedanja Zajedničkog povjerenstva za znanstvenu i tehnološku suradnju. Na šesnaestom zasjedanju Zajedničkog povjerenstva, održanom 10. ožujka 2018. godine u Ljubljani, ova intenzivna suradnja nastavljena je prihvaćanjem 52 nova znanstveno-istraživačka projekta za razdoblje 2018. – 2019. godine. Projekt “Korištenje novih alata u primijenjenim istraživanjima mediteranske poljoprivrede: uvođenje roditeljskog testa u genotipizaciju biljka” kojeg vode prof. dr. sc. Andreja Kutnar (University of Primorska, Faculty of Mathematics, Natural Sciences and Information Technologies – Koper, Slovenia) i dr. sc. Gabriela Vuletin Selak (Institut za jadranske kulture i melioraciju krša, Split) odobren je za financiranje. Prof. dr. sc. Dunja Bandelj, dr. sc. Alenka Baruca Arbeiter, dr. sc. Slavko Perica i dr. sc. Tatjana Klepo, suradnici s UKF projekta sudjeluju i u novom projektu.

The highlighted theme of the 16th Science Festival was “Discoveries”. The Science Festival took place in more than 20 cities in Croatia from the April 16th-21th. The Science Festival in Split offered many events at different locations, and its rich program covered all ages.

As a part of sort lectures presented by scientists from Institute for Adriatic Crops and Karst Reclamation entitled ‘Scientific discoveries in the development of Mediterranean agriculture’, project leader Dr. Gabriela Vuletin Selak, held a presentation „The love life of plants – new approaches to understanding reproductive compatibility of olives“ on April 20 in Marko Marulić city library in Split.

Throughout the lecture, the listeners were introduced to basic knowledge on plant reproduction with emphasis on olive reproductive biology. The importance of understanding the reproduction in plants is highlighted at the beginning because the direct products of this reproduction are fruits and seeds that are the important source of food. The aims, objectives and the significance of the UKF Project were presented as well as the new tools and approaches in the assessment of the compatibility relationships in the olive cultivars.

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Prezentacija na Festivalu znanosti 2018 s ovogodišnjom temom “Otkrića”

U nizu kratkih predavanja djelatnika Instituta za jadranske kulture i melioraciju krša pod zajedničkom temom „Znanstvena otkrića u razvoju mediteranske poljoprivrede“, voditeljica projekta dr. sc. Gabriela Vuletin Selak je 20. travnja 2018. u prostorima Gradske knjižnice Marka Marulića u Splitu održala predavanje na temu „Ljubavni život biljaka – novi pristupi u razumijevanju reproduktivne kompatibilnosti masline“.

Kroz prezentaciju, slušatelje je upoznala s reproduktivnim organima i procesima kod biljaka s naglaskom na reproduktivnu biologiju masline. Na početku predavanja naglašena je važnost istraživanja biljne reproduktivne biologije jer su plodovi i sjemenke koje svakodnevno koristimo u prehrani direktan produkt procesa oprašivanja i oplodnje. Ciljevi i značaj novog UKF projekta koji je vezan uz temu reproduktivne biologije masline su predstavljeni publici zajedno s novim alatima i pristupima u izučavanju reproduktivne kompatibilnosti između sorti maslina.

Ph.D. Gabriela Vuletin Selak held a lecture entitled “New approaches and tools for studying reproductive compatibility in olive – presentation of a new olive-growing project” at the international manifestation of olive growers and olive oil producers NOĆNJAK 2018 held in Vodice from 15 to 18 March 2018.  This is the 20th event organized by the Cooperative Alliance of Dalmatia.

Doc. dr. sc. Gabriela Vuletin Selak održala je predavanje pod nazivom “Novi pristupi i alati u izučavanju reproduktivne kompatibilnosti masline – predstavljanje novog maslinarskog projekta” na međunarodnoj manifestaciji maslinara i uljara NOĆNJAK 2018. održanoj u Vodicama od 15. do 18. ožujka 2018. Ovo je 20. po redu manifestacija koju organizira Zadružni savez Dalmacije.

Nocnjak 2018: program_Nocnjak 2018

PDF_Presentation of the project: UKF project_comp_olea__Nocnjak 2018